![]() ![]() ![]() These steps will result in the DNA sequencing step.After cleavage, DNA fragments was separated using agarose gel electrophoresis.The purpose of this step was to cleave/ligate a functional piece of DNA predictably and precisely (Restriction Enzyme Digestion).Restriction Enzyme Digestion & Gel Electrophoresis.The 16s rDNA sequence of our own two unknown bacteria was amplified.Genomic DNA was extracted from two colonies to identify the bacterial species.After 7 days, we collected the following data for each plate: number of colonies, color, size, shape, texture, form, elevation, and margin.To plate these samples, we used the serial dilution technique.For each collection site, we labeled 3 nutrient agar plates as follows: “ direct count,” “ 1:10,” & “ 1:100“.Collection and Sampling of Environmental Samples at Environmental Education Center.Draft genome sequence of Janthinobacterium lividum strain MTR reveals its mechanism of capnophilic behavior. Valdes, N., Soto, P., Cottet, L., Alarcon, P., Gonzalez, A., Castillo, A., … Tello, M. Janthinobacterium lividum colonizes the skin of some amphibians and confers protection against fungal pathogens. Janthinobacterium lividum is a rod-shaped, Gram-negative, motile, aerobic bacterium, commonly isolated from of soil and water of cold regions. ![]()
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